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EXH AR026670: Static Acute Ceriodaphnia dubia Toxicity Test — Cu, Miller Creek Site Water, Feb 1999

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This document presents results from a 48-hour acute toxicity test conducted in February 1999 for the Port of Seattle, exposing water fleas (Ceriodaphnia dubia) to copper-contaminated water collected from Miller Creek. The test found that all organisms survived at concentrations up to 50 micrograms per liter, while complete mortality occurred at concentrations of 100 micrograms per liter and above. Water quality parameters including pH, dissolved oxygen, and specific conductivity were monitored throughout the test period.

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EXH AR026670. Static acute Ceriodaphnia dubia (water flea) 48-hour toxicity test conducted by Parametrix Toxicology Laboratory for client Port of Seattle. Test material: copper (Cu) in Miller Creek site water (CUSW, μg/L), source WA0024651, sample collected 2/22/99, test dates 2/23/99–2/25/99. Concentrations tested: 0 (control), 1.5625, 3.125, 6.25, 12.5, 25, 50, 100, 150, 200 μg/L. Survival data show 100% survival (proportion alive = 1.00) at concentrations up to 50 μg/L; 0% survival at 100, 150, and 200 μg/L. Water quality parameters recorded include pH, dissolved oxygen (mg/L), and specific conductivity (μS). Test Number 2183. Part of the Third Runway EIS administrative record filed with the Pollution Control Hearings Board (PCHB), State of Washington. Miller Creek, copper, aquatic toxicity, LC50.

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