EXH1113041832

PCHBPollution Control Hearings Board of the State of Washington

I. Whole Efflu_n_t Toxicity Te_tln_ R e2u|atorv Guidance A. Introduction ........................................................................................ I B. WET Testing Requirements in NPDES Permits ......................................... ..... 1 C. Options for Pm-mit_es ..................................................................... •...... 4 H. Whom Effluent Toxicity Test Review A. Introduction ........................................................................................ 7 B. Invalid tests ............................. , ........................................................... 7 1. Failure of EPA Statistical Flowcharts ................................................. 7 2. Appropriate Negative ControLs ......................................................... 9 3. Appropriate Test Termination .......................................................... 9 4. Acceptable Start Counts................................................................ I0 ' 5. Acceptable pH Adjustment ............................................................. 11 6. Randomization ........................................................................... 12 7. Tests Which Fail the Power Standards................................................ 12 C. Other Testing Requirements .................................................................... 13 1. Dechlork-.ation ........................................................................... 13 2. Acute Toxicity Test Duration .......................................................... 13 3. OutdatedEPA Manuals ................................................................. 13 4. Reference Toxicant Tests ............................................................... 13 5. Outliers .................................................................................... 18 6. Excessive Time to Produce a Test Report ............................................ 19 7. Aeration of Test Chambers ............................................................. 19 8. Sporadic Mortalities ..................................................................... 19 9. NOEC Expression ....................................................................... 21 10. Brine Controls ............................................................................ 22 i. Deviations from Protocols and Acceptability Criteria .............................. 22 12. Dual Endpoint Tests ..................................................................... 22 D. Check for Completeness of Report ............................................................ 23 i. Paper Submittals ......................................................................... 23 2. Electronic Submission of Test Data ................................................... 24 HI. Toxicltv Test Report Checkli._t ........................................................................ 25 A. Sample Handling ................................................................................. 25 1. Transfer and Storage .................................................................... 25 2. Holding Time ............................................................................ 26 3. Filtration ................................................................................... 28 4. Aeration ................................................................................... 28 AR 041834 B. Water Quality Measurements ................................................................... 29 i. Purpose .................................................................................... 29 2. Parameters and Schedule ............................................................... 29 C. Toxicity Tests and Species ....................................................................... 31 i. Acute Toxicity Tests…
V V